Operating Method

Connect the Bigloop to the injection valve, first fill it with buffer liquid Then use the injection syringe to put the sample in the Bigloop from the bottom by hand or sample pump.(as show in picture 1.) Push the piston down through infusing buffer solution, , then infuse the sample into the column .The piston is sealed ,which can stop the mixture of buffer and sample.(as show in picture 2.)When the piston move to the bottom place, the buffer liquid will enter into the system through by-pass channel.(as show in picture 3.)